Matrices listed below were spiked with certain level of TRAF5 and the recovery rates were calculated by comparing the measured value to the expected amount of TRAF5 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-96
92
EDTA plasma(n=5)
98-105
100
heparin plasma(n=5)
87-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRAF5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-103%
94-104%
85-104%
EDTA plasma(n=5)
83-99%
83-100%
85-96%
heparin plasma(n=5)
80-99%
80-97%
83-99%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
O00463
Applications
ELISA
Applications Notes
TRAF5 ELISA Kit allows for the in vitro quantitative determination of TRAF5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human TRAF5 (TNF receptor associated factors 5 )ELISA Kit