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Home / ELISA Kit

Human Trace amine-associated receptor 5 (TAAR5) ELISA Kit

SKU : BHE13702672

Trace amine-associated receptor 5 (TAAR5) is a olfactory receptor specific for trimethylamine, a trace amine. Also activated at lower level by dimethylethylamine. Trimethylamine is a bacterial metabolite found in some animal odors, and to humans it is a repulsive odor associated with bad breath and spoiled food. This receptor is probably mediated by the G(s)-class of G-proteins which activate adenylate cyclase.

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Mfr.No.	KTE60455
Description	This Human Trace amine-associated receptor 5 (TAAR5) ELISA Kit employs a two-site sandwich ELISA to quantitate TAAR5.
Alternative Names	TAAR5; MGC138414; MGC138416; PNR; RP11-295F4.5; putative neurotransmitter receptor
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Trace amine-associated receptor 5 microplate Human Trace amine-associated receptor 5 standard Human Trace amine-associated receptor 5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Trace amine-associated receptor 5 (TAAR5) ELISA Kit has high sensitivity and excellent specificity for detection of Human TAAR5. No significant cross-reactivity or interference between Human TAAR5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Trace amine-associated receptor 5 (TAAR5) is a olfactory receptor specific for trimethylamine, a trace amine. Also activated at lower level by dimethylethylamine. Trimethylamine is a bacterial metabolite found in some animal odors, and to humans it is a repulsive odor associated with bad breath and spoiled food. This receptor is probably mediated by the G(s)-class of G-proteins which activate adenylate cyclase.
Notes	This Human Trace amine-associated receptor 5 (TAAR5) ELISA Kit employs a two-site sandwich ELISA to quantitate TAAR5 in samples. An antibody specific for TAAR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAAR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAAR5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAAR5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	9038
Antigen	TAAR5
Tag	TAAR5
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Trace amine-associated receptor 5 (TAAR5) ELISA Kit employs a two-site sandwich ELISA to quantitate TAAR5 in samples. An antibody specific for TAAR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAAR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAAR5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAAR5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702672	KTE60455	48 T	$339		Add to Cart
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