Matrices listed below were spiked with certain level of TPA and the recovery rates were calculated by comparing the measured value to the expected amount of TPA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-101
95
EDTA plasma(n=5)
87-102
93
heparin plasma(n=5)
86-99
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
TPA ELISA Kit allows for the in vitro quantitative determination of TPA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human TPA(Tissue Polypeptide Antigen) ELISA Kit