Human Total P53(Tumor Protein p53) ELISA Kit

Mfr.No.

EH3898

Alternative Names

TP53 ELISA Kit, BCC7 ELISA Kit, LFS1 ELISA Kit, TRP53 ELISA Kit, Antigen NY-CO-13 ELISA Kit, FLJ92943 ELISA Kit, LFS1TRP53 ELISA Kit, p53 ELISA Kit, p53 tumor suppressor ELISA Kit, P53cellular tumor antigen p53 ELISA Kit, Phosphoprotein p53 ELISA Kit, transformation-related protein 53 ELISA Kit, tumor protein p53 ELISA Kit, Tumor suppressor p53 ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

78.125-5000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of TP53 and the recovery rates were calculated by comparing the measured value to the expected amount of TP53 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	87-102	93
EDTA plasma(n=5)	85-103	94
heparin plasma(n=5)	85-101	94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TP53 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	91-102%	90-104%	91-100%
EDTA plasma(n=5)	84-101%	84-98%	91-99%
heparin plasma(n=5)	85-98%	84-98%	80-97%

Sensitivity

< 46.875pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

TP53/BCC7/LFS1/TRP53/Antigen NY-CO-13/FLJ92943/LFS1TRP53/p53/p53 tumor suppressor/P53cellular tumor antigen p53/Phosphoprotein p53/transformation-related protein 53/tumor protein p53/Tumor suppressor p53

Notes

For Research Use Only

UniProt ID	P04637
Antigen	TP53

Applications	ELISA
Applications Notes	Total P53 ELISA Kit allows for the in vitro quantitative determination of TP53 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Journal: Asian Pacific Journal of Cancer Prevention Title: Synergistic Action of 1,2-Epoxy-3 (3- (3,4-dimethoxyphenyl)- 4H-1-benzopiyran-4-on) Propane with Doxorubicin and Cisplatin through Increasing of p53, TIMP-3, and MicroRNA-34a in Cervical Cancer Cell Line (HeLa)

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SKU	Supplier No.	Size	Your price	Quantity
BHE10806187	EH3898	96T	$400		Add to Cart

Human Total P53(Tumor Protein p53) ELISA Kit

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