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Home / ELISA Kit

Human Total IgE ELISA Kit

SKU : BHE13704948

Human Total IgE ELISA Kit quantitates Human IgE in samples.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE62846
Reactivity	Human
Assay Type	Sandwich
Description	Human Total IgE ELISA Kit quantitates Human IgE in samples.
Detection Method	Colorimetric
Calibration Range	10 ng/mL-160 ng/mL
Limit Of Detection	1.0 ng/mL
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Notes	Human Total IgE ELISA Kit quantitates Human IgE in samples. An antigen has been pre-coated onto a microplate. Standards or test samples are added to the wells, incubated and then washed. An antigen-HRP conjugated is then added and incubated. The plate is washed once more and the chromogen solution is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	Human Total IgE ELISA Kit quantitates Human IgE in samples. An antigen has been pre-coated onto a microplate. Standards or test samples are added to the wells, incubated and then washed. An antigen-HRP conjugated is then added and incubated. The plate is washed once more and the chromogen solution is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704948	KTE62846	48 T	$359		Add to Cart
BHE13704948	KTE62846	96 T	$619		Add to Cart
BHE13704948	KTE62846	96 Tx5	$2809		Add to Cart
BHE13704948	KTE62846	96 Tx50	$26209		Add to Cart

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