Human Toll-like Receptor 3

Mfr.No.

E0365Hu

Description

This sandwich kit is for the accurate quantitative detection of human Toll-like Receptor 3 (also known as TLR3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD283;CD283;TLR 3;TLR3;Toll-like receptor 3

Reactivity

Human

Detection Range

50-10000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

103829

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

24.94ng/L

Background

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response.

Gene ID	7098
UniProt ID	O15455
Antigen	TLR3

Research Area

Immunology; Microbiology; Signal Transduction

Protocol

These standard curves of E0365Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6400ng/L	2.29	2.227	2.182
	2.163
3200ng/L	1.294	1.262	1.218
	1.23
1600ng/L	0.807	0.798	0.754
	0.789
800ng/L	0.405	0.447	0.402
	0.488
400ng/L	0.278	0.297	0.253
	0.316
0ng/L	0.043	0.044	0
	0.046

Precision

We measured random samples of E0365Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4903.2	177.35	3.6
Intra-Assay	2	18	1529.3	98.38	6.4
Intra-Assay	3	18	1537.1	107.77	7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102065	E0365Hu	96T	$458		Add to Cart
BHE12102065	E0365Hu	48T	$320		Add to Cart

Human Toll-like Receptor 3 - TLR3 ELISA Kit

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