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Home / ELISA Kit

Human Toll-like receptor 10 (TLR10) ELISA Kit

SKU : BHE13702534

Toll-like receptor 10 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.
The various TLRs exhibit different patterns of expression. This gene is most highly expressed in lymphoid tissues such as spleen, lymph node, thymus, and tonsil. Its exact function is not known. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

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Mfr.No.	KTE60316
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Toll-like receptor 10 (TLR10) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR10.
Alternative Names	TLR10; CD290; MGC104967; MGC126398; MGC126399;
Detection Method	Colorimetric
Kit Components	Human Toll-like receptor 10 microplate Human Toll-like receptor 10 standard Human Toll-like receptor 10 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Toll-like receptor 10 (TLR10) ELISA Kit has high sensitivity and excellent specificity for detection of Human TLR10. No significant cross-reactivity or interference between Human TLR10 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Toll-like receptor 10 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular pattes (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different pattes of expression. This gene is most highly expressed in lymphoid tissues such as spleen, lymph node, thymus, and tonsil. Its exact function is not known. Multiple alteatively spliced transcript variants encoding the same protein have been found for this gene.
Notes	This Human Toll-like receptor 10 (TLR10) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR10 in samples. An antibody specific for TLR10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLR10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	81793

Applications	ELISA
Applications Notes	This Human Toll-like receptor 10 (TLR10) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR10 in samples. An antibody specific for TLR10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLR10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702534	KTE60316	48 T	$339		Add to Cart
BHE13702534	KTE60316	96 T	$589		Add to Cart
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BHE13702534	KTE60316	96 Tx50	$24569		Add to Cart

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