Human Toll-like Receptor 1

Mfr.No.

E0363Hu

Description

This sandwich kit is for the accurate quantitative detection of human Toll-like Receptor 1 (also known as TLR1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD281;TIL;TLR 1;TLR1;Toll/interleukin-1 receptor-like protein;Toll-like receptor 1

Reactivity

Human

Detection Range

0.2-60ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

90291

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.09ng/ml

Background

Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed: 21078852). Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed: 16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Gene ID	7096
UniProt ID	Q15399
Antigen	TLR1

Research Area

Immunology

Protocol

These standard curves of E0363Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.246	2.301	2.271
	2.355
20ng/ml	1.363	1.408	1.379
	1.454
10ng/ml	0.603	0.658	0.629
	0.713
5ng/ml	0.385	0.348	0.319
	0.311
2.5ng/ml	0.154	0.139	0.11
	0.125
0ng/ml	0.037	0.03	0
	0.022

Precision

We measured random samples of E0363Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	18.6	0.66	3.6
Intra-Assay	2	18	6.7	0.28	4.2
Intra-Assay	3	18	11.4	0.45	4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102063	E0363Hu	96T	$458		Add to Cart
BHE12102063	E0363Hu	48T	$320		Add to Cart

Human Toll-like Receptor 1 - TLR1 ELISA Kit

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