Human Toll Interacting Protein

Mfr.No.

E6281Hu

Sensitivity

5.18ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

IL-1RAcPIP;Toll-interacting protein;TOLLIP

Molecular Weight

30282

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6281Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	375.84	21.21	5.64
Intra-Assay	2	18	712.48	33.79	4.74
Intra-Assay	3	18	241.94	13.4	5.54

Background

Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity (PubMed:11751856). Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

Protocol

These standard curves of E6281Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.077	2.147	2.094
	2.218
600ng/L	1.34	1.443	1.39
	1.546
300ng/L	0.908	0.862	0.809
	0.817
150ng/L	0.547	0.51	0.457
	0.472
75ng/L	0.236	0.237	0.184
	0.239
0ng/L	0.064	0.053	0
	0.042

Gene ID	54472
UniProt ID	Q9H0E2

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107643	E6281Hu	48T	$320		Add to Cart
BHE12107643	E6281Hu	96T	$458		Add to Cart

Human Toll Interacting Protein - TOLLIP ELISA Kit

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