Human TNFSF9(Tumor Necrosis Factor Ligand Superfamily, Member 9) ELISA Kit

Mfr.No.

ELK4364

Sensitivity

2.7 pg/mL

Detection Range

7.82-500 pg/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

4-1BB-L; Receptor 4-1BB Ligand; Homolog Of Mouse 4-1BB-L

Recovery

Matrices listed below were spiked with certain level of recombinant TNFSF9 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFSF9 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-93%	86%
EDTA plasma(n=5)	87-97%	90%
Heparin plasma(n=5)	88-102%	95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFSF9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	87-98%	92-105%	83-92%	89-98%
EDTA plasma(n=5)	83-97%	86-97%	90-98%	80-93%
Heparin plasma(n=5)	87-98%	92-104%	82-96%	95-102%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
500.00	2.132	2.024
250.00	1.565	1.457
125.00	1.217	1.109
62.50	0.892	0.784
31.25	0.509	0.401
15.63	0.315	0.207
7.82	0.179	0.071
0.00	0.108	0.000

Limit Of Detection

500 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFSF9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFSF9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFSF9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNFSF9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P41273

Applications	ELISA
Research Area	Tumor immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15202159	ELK4364	48T	$320		Add to Cart
BHE15202159	ELK4364	96T	$458		Add to Cart

Human TNFSF9(Tumor Necrosis Factor Ligand Superfamily, Member 9) ELISA Kit

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