Human TNFRSF7/CD27 ELISA Kit PicoKine®

Mfr.No.

EK0698

Sensitivity

<1 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.025	0.121	0.218	0.369	0.719	1.036	1.742	2.097

Description

Human TNFRSF7/CD27 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD27 in cell culture supeatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CD27 antigen;CD27L receptor;T-cell activation antigen CD27;T14;Tumor necrosis factor receptor superfamily member 7;CD27;CD27;TNFRSF7;

Molecular Weight

29137 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: T21-I192

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TNFRSF7

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	103	5108	1421	108	586	1478
Standard deviation	6.79	27.37	92.36	7.60	35.74	121.19
CV (%)	6.6%	4.6%	6.5%	7.6%	6.1%	8.2%

Protein Function

Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Background

CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	103	115	112	118	112	5.61	5%
Sample 2	595	611	638	634	619	17.5	2.8%
Sample 3	1421	1571	1362	1358	1428	86.24	6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	939
Gene Name	CD27
UniProt ID	P26842
Protein Name	CD27 antigen
Tissue Specificity	Found in most T-lymphocytes.
Sequence	Contains 3 TNFR-Cys repeats.

Applications	ELISA
Research Area	Adaptive Immunity,Cancer,Cytokines,Hematopoietic Progenitors,Immunology,Innate Immunity,Lymphoid,Stem Cells,T Cells,T Lymphocytic Lineage,TNF Superfamily,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000572	EK0698	96 wells/kit	$499		Add to Cart

Human TNFRSF7/CD27 ELISA Kit PicoKine®

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