Matrices listed below were spiked with certain level of TNFRSF21 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFRSF21 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-97
95
EDTA plasma(n=5)
92-103
98
heparin plasma(n=5)
91-100
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFRSF21 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
100-103%
86-94%
EDTA plasma(n=5)
82-98%
85-94%
83-94%
heparin plasma(n=5)
81-99%
82-100%
85-97%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
O75509
Applications
ELISA
Applications Notes
TNFRSF21 ELISA Kit allows for the in vitro quantitative determination of TNFRSF21 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human TNFRSF21(Tumor necrosis factor receptor superfamily member 21) ELISA Kit