Human TNFRSF19/TROY ELISA Kit PicoKine®

Mfr.No.

EK1584

Sensitivity

<12 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.024	0.075	0.139	0.252	0.474	0.81	1.494	2.081

Description

Human TNFRSF19/TROY ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF19 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 19;TRADE;Toxicity and JNK inducer;TNFRSF19;TAJ, TROY;UNQ1888/PRO4333;

Molecular Weight

46015 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E30-L170

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TNFRSF19

Subcellular Localization

Membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	184	541	1334	198	536	1337
Standard deviation	13.43	36.24	88.04	16.83	43.95	112.3
CV (%)	7.3%	6.7%	6.6%	8.5%	8.2%	8.4%

Protein Function

Can mediate activation of JNK and NF-kappa-B. May promote caspase-independent cell death.

Background

Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY, is a human gene. It is mapped to 13q12.11-q12.3. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alteatively spliced transcript variants encoding distinct isoforms have been described.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	184	205	190	190	192	7.75	4%
Sample 2	541	552	500	518	527	20.17	3.8%
Sample 3	1334	1468	1351	1411	1391	52.86	3.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	55504
Gene Name	TNFRSF19
UniProt ID	Q9NS68
Protein Name	Tumor necrosis factor receptor superfamily member 19
Tissue Specificity	Highly expressed in prostate. Detected at lower levels in thymus, spleen, testis, uterus, small intestine, colon and peripheral blood leukocytes.

Applications	ELISA
Research Area	Apoptosis,Associated Proteins,Cancer,Cell Biology,Growth Factors,Growth Factors/Hormones,Intracellular,Neurogenesis,Neurology Process,Neuroscience,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001267	EK1584	96 wells/kit	$499		Add to Cart

Human TNFRSF19/TROY ELISA Kit PicoKine®

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