Human TNFRSF17/BCMA ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0661

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.011	0.154	0.256	0.452	0.734	1.225	1.678	1.984

Description

Human TNFRSF17/BCMA ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human TNFRSF17 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA).

Alternative Names

Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; CD269; TNFRSF17; BCM; BCMA

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-A54

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TNFRSF17/BCMA

Subcellular Localization

Cell membrane; Perinuclear Golgi-like structures.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	134	642	1286	158	614	1358
Standard deviation	4.69	35.95	61.73	6.64	41.14	73.33
CV (%)	3.5%	5.6%	4.8%	4.2%	6.7%	5.4%

Protein Function

Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.

Background

TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	608
Gene Name	TNFRSF17
UniProt ID	Q02223
Tissue Specificity	Expressed in mature B-cells, but not in T- cells or monocytes.

Applications	ELISA
Research Area	Antiviral Signaling,Cancer,Growth Factors,Growth Factors/Hormones,Immune System Diseases,Immunology,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000039	EZ0661	5 plates/kit	$500		Add to Cart

Human TNFRSF17/BCMA ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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