Human Tnfaip3-interacting Protein 3

Mfr.No.

E6280Hu

Alternative Names

A20-binding inhibitor of NF-kappa-B activation 3;ABIN-3;LIND;Listeria-induced gene protein;TNFAIP3-interacting protein 3;TNIP3

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

38943

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.06ng/L

Background

Binds to zinc finger protein TNFAIP3 and inhibits NF-kappa-B activation induced by tumor necrosis factor, Toll-like receptor 4 (TLR4), interleukin-1 and 12-O-tetradecanoylphorbol-13-acetate. Overexpression inhibits NF-kappa-B-dependent gene expression in response to lipopolysaccharide at a level downstream of TRAF6 and upstream of IKBKB. NF-kappa-B inhibition is independent of TNFAIP3 binding.

Gene ID	79931
UniProt ID	Q96KP6
Antigen	TNIP3

Research Area

Signal Transduction; Cardiovascular

Protocol

These standard curves of E6280Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.13	2.146	2.102
	2.162
800ng/L	1.38	1.46	1.416
	1.541
400ng/L	0.944	0.948	0.904
	0.951
200ng/L	0.474	0.485	0.44
	0.495
100ng/L	0.297	0.289	0.244
	0.28
0ng/L	0.051	0.044	0
	0.038

Precision

We measured random samples of E6280Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	339.26	19.76	5.83
Intra-Assay	2	18	1066.67	55.04	5.16
Intra-Assay	3	18	279.89	18.72	6.69

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107642	E6280Hu	48T	$320		Add to Cart
BHE12107642	E6280Hu	96T	$458		Add to Cart

Human Tnfaip3-interacting Protein 3 - TNIP3 ELISA Kit

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