Human TNF Beta/Lymphotoxin-alpha ELISA Kit PicoKine®

Mfr.No.

EK0584

Sensitivity

<5 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.038	0.180	0.293	0.450	0.737	1.345	1.668	1.926

Description

Human TNF Beta ELISA Kit PicoKine® (96 Tests). Quantitate Human LTA in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Lymphotoxin-alpha;LT-alpha;TNF-beta;Tumor necrosis factor ligand superfamily member 1;LTA;TNFB, TNFSF1;

Molecular Weight

22297 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L35-L205

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TNF beta

Subcellular Localization

Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	165	487	1791	179	521	1896
Standard deviation	11.71	32.14	127.16	13.06	38.55	157.36
CV (%)	7.1%	6.6%	7.1%	7.3%	7.4%	8.3%

Protein Function

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.

Background

Tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin-alpha (LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35% identity and 50% homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	165	162	154	142	155	8.89	5.7%
Sample 2	487	454	458	413	453	26.37	5.8%
Sample 3	1791	1778	1760	1730	1764	22.88	1.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4049
Gene Name	LTA
UniProt ID	P01374
Protein Name	Lymphotoxin-alpha
Sequence	Belongs to the tumor necrosis factor family.

Applications	ELISA
Research Area	Biochemicals,Chemical Type,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipid Signaling,Metabolic Signaling Pathways,Metabolism,Pathways and Processes

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000534	EK0584	96 wells/kit	$499		Add to Cart

Human TNF Beta/Lymphotoxin-alpha ELISA Kit PicoKine®

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