Human TNF alpha converting enzyme,TACE ELISA Kit

Human TNF alpha converting enzyme,TACE ELISA Kit

Reviews of Human TNF alpha converting enzyme,TACE ELISA Kit

Rate it now..!!

Rate it now..!!

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Mfr.No.

CSB-E09315h

Alternative Names

ADAM metallopeptidase domain 17, ADAM18, CD156B, CSVP, MGC71942, TACE, ADAM metallopeptidase domain 18, TNF-alpha converting enzyme, a disintegrin and metalloprotease domain 17, snake venom-like protease, tumor necrosis factor, alpha, TNF alpha converting enzyme,TACE

Reactivity

Human

Detection Range

62.5 pg/mL-4000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	97	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	84-101
1:2	Average %	94
	Range %	90-100
1:4	Average %	96
	Range %	91-103
1:8	Average %	94
	Range %	86-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
250	2.712	2.892	2.802	2.614
125	2.369	2.317	2.343	2.155
62.5	1.568	1.604	1.586	1.398
31.25	0.877	0.888	0.883	0.695
15.6	0.558	0.562	0.560	0.372
7.8	0.371	0.384	0.378	0.190
3.9	0.276	0.286	0.281	0.093
0	0.188	0.187	0.188	?

Species

Homo sapiens

Sensitivity

15.6 pg/mL

Limit Of Detection

450 nm

UniProt ID	P78536
Antigen	ADAM metallopeptidase domain 17

Applications

ELISA

Research Area

ADAM17

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10504597	CSB-E09315h	96T	$595	Add to Cart
BHE10504597	CSB-E09315h	5x96T	$2856	Add to Cart
BHE10504597	CSB-E09315h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.