Human TNF alpha converting enzyme,TACE ELISA Kit

Human TNF alpha converting enzyme,TACE ELISA Kit

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Mfr.No.

CSB-E09315h

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ADAM metallopeptidase domain 17, ADAM18, CD156B, CSVP, MGC71942, TACE, ADAM metallopeptidase domain 18, TNF-alpha converting enzyme, a disintegrin and metalloprotease domain 17, snake venom-like protease, tumor necrosis factor, alpha, TNF alpha converting enzyme,TACE

Recovery

The recovery of rat VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	97	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	84-101
1:2	Average %	94
	Range %	90-100
1:4	Average %	96
	Range %	91-103
1:8	Average %	94
	Range %	86-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
250	2.712	2.892	2.802	2.614
125	2.369	2.317	2.343	2.155
62.5	1.568	1.604	1.586	1.398
31.25	0.877	0.888	0.883	0.695
15.6	0.558	0.562	0.560	0.372
7.8	0.371	0.384	0.378	0.190
3.9	0.276	0.286	0.281	0.093
0	0.188	0.187	0.188	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P78536

Applications	ELISA
Research Area	ADAM17

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10504597	CSB-E09315h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.