Matrices listed below were spiked with certain level of TMEM173 and the recovery rates were calculated by comparing the measured value to the expected amount of TMEM173 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
95
EDTA plasma(n=5)
85-105
92
heparin plasma(n=5)
86-101
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TMEM173 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-98%
85-105%
85-102%
EDTA plasma(n=5)
89-101%
87-98%
83-95%
heparin plasma(n=5)
80-93%
84-97%
81-99%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q86WV6
Antigen
TMEM173
Applications
ELISA
Applications Notes
TMEM173 ELISA Kit allows for the in vitro quantitative determination of TMEM173 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human TMEM173(Transmembrane protein 173) ELISA Kit