Matrices listed below were spiked with certain level of TMEM127 and the recovery rates were calculated by comparing the measured value to the expected amount of TMEM127 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
95
EDTA plasma(n=5)
87-99
95
heparin plasma(n=5)
86-102
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TMEM127 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-104%
85-103%
87-101%
EDTA plasma(n=5)
90-100%
87-97%
82-99%
heparin plasma(n=5)
86-97%
87-97%
83-100%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
O75204
Antigen
TMEM127
Applications
ELISA
Applications Notes
TMEM127 ELISA Kit allows for the in vitro quantitative determination of TMEM127 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human TMEM127(Transmembrane protein 127) ELISA Kit