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Home / ELISA Kit

Human TM2 domain-containing protein 1 (TM2D1) ELISA Kit

SKU : BHE13702530

TM2D1 is a beta-amyloid peptide-binding protein. It contains a structural module related to that of the seven transmembrane domain G protein-coupled receptor superfamily and known to be important in heterotrimeric G protein activation. Beta-amyloid peptide has been established to be a causative factor in neuron death and the consequent diminution of cognitive abilities observed in Alzheimer's disease. This protein may be a target of neurotoxic beta-amyloid peptide, and may mediate cellular vulnerability to beta-amyloid peptide toxicity through a G protein-regulated program of cell death.BBP bound the 42-amino acid form of human beta-amyloid peptide in vitro with high affinity and specificity. Expression of a signaling-deficient dominant-negative BBP mutant suppressed sensitivity of human Ntera-2 neurons to beta-amyloid-mediated toxicity.

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Mfr.No.	KTE60312
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human TM2 domain-containing protein 1 (TM2D1) ELISA Kit employs a two-site sandwich ELISA to quantitate TM2D1.
Alternative Names	TM2D1; BBP; beta-amyloid binding protein
Detection Method	Colorimetric
Kit Components	Human TM2 domain-containing protein 1 microplate Human TM2 domain-containing protein 1 standard Human TM2 domain-containing protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human TM2 domain-containing protein 1 (TM2D1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TM2D1. No significant cross-reactivity or interference between Human TM2D1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TM2D1 is a beta-amyloid peptide-binding protein. It contains a structural module related to that of the seven transmembrane domain G protein-coupled receptor superfamily and known to be important in heterotrimeric G protein activation. Beta-amyloid peptide has been established to be a causative factor in neuron death and the consequent diminution of cognitive abilities observed in Alzheimer's disease. This protein may be a target of neurotoxic beta-amyloid peptide, and may mediate cellular vulnerability to beta-amyloid peptide toxicity through a G protein-regulated program of cell death.BBP bound the 42-amino acid form of human beta-amyloid peptide in vitro with high affinity and specificity. Expression of a signaling-deficient dominant-negative BBP mutant suppressed sensitivity of human Ntera-2 neurons to beta-amyloid-mediated toxicity.
Notes	This Human TM2 domain-containing protein 1 (TM2D1) ELISA Kit employs a two-site sandwich ELISA to quantitate TM2D1 in samples. An antibody specific for TM2D1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM2D1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM2D1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM2D1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	83941

Applications	ELISA
Applications Notes	This Human TM2 domain-containing protein 1 (TM2D1) ELISA Kit employs a two-site sandwich ELISA to quantitate TM2D1 in samples. An antibody specific for TM2D1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM2D1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM2D1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM2D1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702530	KTE60312	48 T	$339		Add to Cart
BHE13702530	KTE60312	96 T	$589		Add to Cart
BHE13702530	KTE60312	96 Tx5	$2629		Add to Cart
BHE13702530	KTE60312	96 Tx50	$24569		Add to Cart

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