Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of TLR8 and the recovery rates were calculated by comparing the measured value to the expected amount of TLR8 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
96
EDTA plasma(n=5)
86-102
95
heparin plasma(n=5)
87-105
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TLR8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-104%
90-104%
93-101%
EDTA plasma(n=5)
85-96%
84-98%
85-99%
heparin plasma(n=5)
83-98%
86-93%
87-96%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q9NR97
Antigen
TLR8
Applications
ELISA
Applications Notes
TLR8 ELISA Kit allows for the in vitro quantitative determination of TLR8 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human TLR8 (Toll-like receptor 8) ELISA Kit