Human TLR3/Toll-like receptor 3 ELISA Kit PicoKine®

Mfr.No.

EK0907

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.079	0.174	0.258	0.407	0.684	1.056	1.570	2.074

Description

Human TLR3/Toll-like receptor 3 ELISA Kit PicoKine® (96 Tests). Quantitate Human TLR3 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Toll-like receptor 3;CD283;TLR3;

Molecular Weight

103829 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: K27-S711

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TLR3

Subcellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome membrane.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	290	1771	5327	301	1733	5141
Standard deviation	14.21	125.74	292.98	18.96	152.5	374.9%9
CV (%)	4.9%	7.1%	5.5%	8.8%%	8.8%	7.3%

Protein Function

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular pattes specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response. .

Background

Toll-like receptor 3 (TLR3) also known as CD283 (cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene was mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 specifically recognizes double-stranded RNA (dsRNA) and induces multiple intracellular events responsible for innate antiviral immunity against a number of viral infections.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	290	307	297	293	296	6.41	2.1%
Sample 2	1771	1687	1955	1718	1782	103.88	5.8%
Sample 3	5327	6029	6362	5287	5751	459.8	7.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7098
Gene Name	TLR3
UniProt ID	O15455
Protein Name	Toll-like receptor 3
Tissue Specificity	Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. .
Sequence	Belongs to the Toll-like receptor family.

Applications	ELISA
Research Area	Chemokines,Host-Virus Interaction,Immune System Diseases,Immunoglobulins,Immunology,Innate Immunity,Interspecies Interaction,Macrophage/Inflammation,Microbiology,NFKB Pathway,Nuclear Signaling,Receptors,Signal Transduction,Signaling Pathway,TLR Signaling

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000688	EK0907	96 wells/kit	$499		Add to Cart

Human TLR3/Toll-like receptor 3 ELISA Kit PicoKine®

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