Human TLR1/TIL ELISA Kit PicoKine®

Mfr.No.

EK1129

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.059	0.188	0.250	0.421	0.755	1.400	1.931	2.311

Description

Human TLR1 / TIL ELISA Kit PicoKine® (96 Tests). Quantitate Human TLR1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Toll-like receptor 1;Toll/interleukin-1 receptor-like protein;TIL;CD281;TLR1;KIAA0012;

Molecular Weight

90291 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S22-N578

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TLR1

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein . Cytoplasmic vesicle, phagosome membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	204	1142	6099	218	1081	6218
Standard deviation	11.22	78.79	378.13	16.2%1	77.83	472.56
CV (%)	5.5%	6.9%	6.2%	7.3%	7.2%	7.6%

Protein Function

Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). .

Background

TLR1 is a member of the Toll-like receptor family (TLR) of patte recognition receptors of the innate immune system. TIR1 gene was mapped to 4p14 by fluorescence in situ hybridization. TLR1 recognizes pathogen-associated molecular patte with a specificity for gram-positive bacteria. TLR1 has also been designated as CD281 (cluster of differentiation 281). TLR1 interacts with TLR2 to recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	204	208	203	192	201	5.93	2.9%
Sample 2	1142	1045	1032	1050	1067	43.65	4%
Sample 3	6099	6066	5527	6006	5924	231.9	3.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7096
Gene Name	TLR1
UniProt ID	Q15399
Protein Name	Toll-like receptor 1
Tissue Specificity	Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.
Sequence	Belongs to the Toll-like receptor family.

Applications	ELISA
Research Area	Adapters,Immunoglobulins,Immunology,Innate Immunity,Macrophage/Inflammation,NFKB Pathway,Nuclear Signaling,Receptors,Signal Transduction,Signaling Pathway,TLR Signaling,Transmembrane

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000781	EK1129	96 wells/kit	$750		Add to Cart

Human TLR1/TIL ELISA Kit PicoKine®

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