Human titin antibody(IgG) ELISA Kit

Mfr.No.

CSB-E13356h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

titin antibody(IgG)

Recovery

The recovery of dog CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	96-108
EDTA plasma (n=4)	99	91-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	105
	Range %	97-110
1:2	Average %	101
	Range %	95-106
1:4	Average %	96
	Range %	87-101
1:8	Average %	102
	Range %	97-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	IgG

Storage	Store at 2-8°C. Please refer to protocol.

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