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Home / ELISA Kit

Human Tissue inhibitors of metalloproteinase 2 (TIMP2) ELISA Kit

SKU : BHE13702544

TIMP2, is a member of the TIMP family. The proteins are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. In melanocytic cells TIMP2 gene expression may be regulated by MITF.

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Mfr.No.	KTE60326
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tissue inhibitors of metalloproteinase 2 (TIMP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP2.
Alternative Names	TIMP2; CSC-21K; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2
Detection Method	Colorimetric
Kit Components	Human Tissue inhibitors of metalloproteinase 2 microplate Human Tissue inhibitors of metalloproteinase 2 standard Human Tissue inhibitors of metalloproteinase 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tissue inhibitors of metalloproteinase 2 (TIMP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TIMP2. No significant cross-reactivity or interference between Human TIMP2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TIMP2, is a member of the TIMP family. The proteins are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. In melanocytic cells TIMP2 gene expression may be regulated by MITF.
Notes	This Human Tissue inhibitors of metalloproteinase 2 (TIMP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP2 in samples. An antibody specific for TIMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7077

Applications	ELISA
Applications Notes	This Human Tissue inhibitors of metalloproteinase 2 (TIMP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP2 in samples. An antibody specific for TIMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702544	KTE60326	48 T	$359		Add to Cart
BHE13702544	KTE60326	96 T	$619		Add to Cart
BHE13702544	KTE60326	96 Tx5	$2809		Add to Cart
BHE13702544	KTE60326	96 Tx50	$26209		Add to Cart

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