Human Tissue Inhibitor Of Matrix Metalloprotease-1 (TIMP-1) ELISA KIT

Human Tissue Inhibitor Of Matrix Metalloprotease-1 (TIMP-1) ELISA KIT

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Mfr.No.

CSB-E08003h

Sensitivity

0.434 ng/mL

Detection Range

0.39 ng/mL-25 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

TIMP metallopeptidase inhibitor 1, RP1-230G1.3, CLGI, EPA, EPO, FLJ90373, HCI, TIMP, erythroid potentiating activity, fibroblast collagenase inhibitor, tissue inhibitor of metalloproteinase 1

Recovery

The recovery of dog CIC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-101
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog CIC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	85-100
1:200	Average %	89
	Range %	83-99
1:400	Average %	97
	Range %	90-112
1:800	Average %	93
	Range %	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
1200	0.148	0.152	0.150
300	0.420	0.396	0.408
37.5	0.702	0.693	0.698
4.68	1.233	1.144	1.189
2.34	1.702	1.744	1.723
0	2.406	2.455	2.431

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P01033

Applications	ELISA
Research Area	TIMP1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504585	CSB-E08003h	96T	$450	Add to Cart
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BHE10504585	CSB-E08003h	10x96T	$4275	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.