Human Tissue Factor/F3 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0928

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.035	0.09	0.155	0.254	0.479	0.914	1.446	2.308

Description

Human Tissue Factor/F3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human F3 in cell culture supeatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine.

Alternative Names

Tissue factor;TF;Coagulation factor III;Thromboplastin;CD142;F3;

Molecular Weight

33068 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S33-E251

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Tissue Factor/F3

Subcellular Localization

Isoform 1: Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma, Cell lysates, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	106	285	593	162	321	630
Standard deviation	3.71	15.39	29.06	9.07	22.15	36.54
CV (%)	3.5%	5.4%	4.9%	5.6%	6.9%	5.8%

Protein Function

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The (TF:VIIa) complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. .

Background

Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. An incorrect synonym is thromboplastin. the F3 gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	2152
Gene Name	F3
UniProt ID	P13726
Protein Name	Tissue factor
Tissue Specificity	Lung, placenta and pancreas. .
Sequence	Belongs to the tissue factor family.

Applications	ELISA
Research Area	Cardiovascular

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000020	EZ0928	5 plates/kit	$500		Add to Cart

Human Tissue Factor/F3 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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