Human TIPE2(TNF alpha-induced protein 8-like protein 2) ELISA Kit

Mfr.No.

ELK8820

Alternative Names

TIPE2; TNF alpha-induced protein 8-like protein 2; TNFAIP8-like protein 2; TNFAIP8L2; TP8L2_HUMAN; Tumor necrosis factor alpha induced protein 8 like 2; Tumor necrosis factor alpha-induced protein 8-like protein 2

Reactivity

Human

Detection Range

15.63-1000 pg/mL

Sample Type

Tissue homogenates, Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant TIPE2 and the recovery rates were calculated by comparing the measured value to the expected amount of TIPE2 in samples.

Matrix	Recovery range	Average
serum(n=5)	93-107%	100%
EDTA plasma(n=5)	87-95%	91%
Heparin plasma(n=5)	87-99%	93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TIPE2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	81-94%	96-105%	92-103%	82-96%
EDTA plasma(n=5)	87-93%	95-106%	88-103%	81-98%
Heparin plasma(n=5)	78-90%	91-99%	97-103%	87-98%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.096	2.001
500.00	1.624	1.529
250.00	1.053	0.958
125.00	0.877	0.782
62.50	0.496	0.401
31.25	0.357	0.262
15.63	0.216	0.121
0.00	0.095	0.000

Sensitivity

5.9 pg/mL

Limit Of Detection

1000 pg/mL

UniProt ID	Q6P589
Antigen	TIPE2
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TIPE2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TIPE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TIPE2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TIPE2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Infection immunity
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200864	ELK8820	48T	$412		Add to Cart
BHE15200864	ELK8820	96T	$588		Add to Cart

Human TIPE2(TNF alpha-induced protein 8-like protein 2) ELISA Kit

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