Human Thyroxine-Binding Globulin,TBG ELISA Kit

Mfr.No.

CSB-E09501h

Sensitivity

12.5 ng/mL

Detection Range

12.5 ng/mL-200 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7, RP1-82J11.2, TBG, OTTHUMP00000023779, alpha-1 antiproteinase, antitrypsin, serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7, serine (or cysteine) prote

Recovery

The recovery of human CLEC7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	96-104
EDTA plasma (n=4)	96	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

84-95

1:2

Average %

Range %

85-95

1:4

Average %

Range %

92-101

1:8

Average %

Range %

89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.246	2.297	2.272	2.154
1000	1.821	1.862	1.842	1.724
500	1.315	1.336	1.326	1.208
250	0.887	0.874	0.881	0.763
125	0.453	0.463	0.458	0.340
62.5	0.322	0.342	0.332	0.214
31.25	0.262	0.254	0.258	0.140
0	0.119	0.117	0.118	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P05543

Applications	ELISA
Research Area	SERPINA7

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504577	CSB-E09501h	96T	$790	Add to Cart
BHE10504577	CSB-E09501h	5x96T	$3792	Add to Cart
BHE10504577	CSB-E09501h	10x96T	$7505	Add to Cart

Human Thyroxine-Binding Globulin,TBG ELISA Kit

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