Human thymus activation regulated chemokine,TARC ELISA Kit

Human thymus activation regulated chemokine,TARC ELISA Kit

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Mfr.No.

CSB-E09257h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

chemokine (C-C motif) ligand 17, A-152E5.3, ABCD-2, MGC138271, MGC138273, SCYA17, TARC, T cell-directed CC chemokine, small inducible cytokine A17, small inducible cytokine subfamily A (Cys-Cys), member 17, thymus and activation-regul

Recovery

The recovery of mouse retinoic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-103
EDTA plasma (n=4)	94	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse retinoic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	92
	Range %	87-97
1:40	Average %	90
	Range %	85-94
1:80	Average %	84
	Range %	80-88
1:160	Average %	89
	Range %	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
400	0.113	0.110	0.112
200	0.257	0.246	0.252
100	0.451	0.437	0.444
50	0.709	0.726	0.718
25	1.103	1.081	1.092
0	1.672	1.629	1.651

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q92583
Tissue Specificity	Expressed at high levels in thymus and at low levels in the lung, colon and small intestine.

Applications	ELISA
Research Area	CCL17

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10504566	CSB-E09257h	10x96T	$4446	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.