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Home / ELISA Kit

Human Thymidylate synthetase (TS) ELISA Kit

SKU : BHE13702345

Tuberous sclerosis protein 1, also known as TSC1 or hamartin, This peripheral membrane protein was implicated as a tumor suppressor. It may be also involved in vesicular transport and docking, in complex with TSC2.Defects in this gene may cause tuberous sclerosis, due to a functional impairment of the hamartin-tuberin complex. Defects in TSC1 may also be a cause of focal cortical dysplasia.In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling.

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Mfr.No.	KTE60126
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Thymidylate synthetase (TS) ELISA Kit employs a two-site sandwich ELISA to quantitate TS.
Alternative Names	TS
Detection Method	Colorimetric
Kit Components	Human Thymidylate synthetase microplate Human Thymidylate synthetase standard Human Thymidylate synthetase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Thymidylate synthetase (TS) ELISA Kit has high sensitivity and excellent specificity for detection of Human TS. No significant cross-reactivity or interference between Human TS and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tuberous sclerosis protein 1, also known as TSC1 or hamartin, This peripheral membrane protein was implicated as a tumor suppressor. It may be also involved in vesicular transport and docking, in complex with TSC2.Defects in this gene may cause tuberous sclerosis, due to a functional impairment of the hamartin-tuberin complex. Defects in TSC1 may also be a cause of focal cortical dysplasia.In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling.
Notes	This Human Thymidylate synthetase (TS) ELISA Kit employs a two-site sandwich ELISA to quantitate TS in samples. An antibody specific for TS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Thymidylate synthetase (TS) ELISA Kit employs a two-site sandwich ELISA to quantitate TS in samples. An antibody specific for TS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702345	KTE60126	48 T	$339		Add to Cart
BHE13702345	KTE60126	96 T	$589		Add to Cart
BHE13702345	KTE60126	96 Tx5	$2629		Add to Cart
BHE13702345	KTE60126	96 Tx50	$24569		Add to Cart

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