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Home / ELISA Kit

Human Thymic stromal lymphopoietin (TSLP) ELISA Kit

SKU : BHE13702340

The cytokine thymic stromal lymphopoietin (TSLP) drives immature B cell development in vitro and may regulate T helper type 2 responses.Thymic stromal lymphopoietin, also known as TSLP, is a human gene.This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c dendritic cells.
Alternative splicing of this gene results in two transcript variants. TSLP is an interleukin (IL)-7-like cytokine produced by epithelial cells and triggers dendritic cell-mediated Th2 type allergic inflammatory responses.

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Mfr.No.	KTE60121
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Thymic stromal lymphopoietin (TSLP) ELISA Kit employs a two-site sandwich ELISA to quantitate TSLP.
Alternative Names	TSLP
Detection Method	Colorimetric
Kit Components	Human Thymic stromal lymphopoietin microplate Human Thymic stromal lymphopoietin standard Human Thymic stromal lymphopoietin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Thymic stromal lymphopoietin (TSLP) ELISA Kit has high sensitivity and excellent specificity for detection of Human TSLP. No significant cross-reactivity or interference between Human TSLP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The cytokine thymic stromal lymphopoietin (TSLP) drives immature B cell development in vitro and may regulate T helper type 2 responses.Thymic stromal lymphopoietin, also known as TSLP, is a human gene.This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c dendritic cells. Alternative splicing of this gene results in two transcript variants. TSLP is an interleukin (IL)-7-like cytokine produced by epithelial cells and triggers dendritic cell-mediated Th2 type allergic inflammatory responses.
Notes	This Human Thymic stromal lymphopoietin (TSLP) ELISA Kit employs a two-site sandwich ELISA to quantitate TSLP in samples. An antibody specific for TSLP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTSLP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSLP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSLP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	85480

Applications	ELISA
Applications Notes	This Human Thymic stromal lymphopoietin (TSLP) ELISA Kit employs a two-site sandwich ELISA to quantitate TSLP in samples. An antibody specific for TSLP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTSLP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSLP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSLP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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