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Home / ELISA Kit

Human Thrombus precursor protein (TpP) ELISA Kit

SKU : BHE13702365

Thrombus precursor protein (TpP), which measures soluble fibrin, is a marker of active thrombosis. Patients with ACS who present early after symptom onset have been shown to have significantly higher levels of TpP as compared to controls. Several smaller studies have evaluated TpP as a diagnostic tool and found that TpP appears to offer useful information for patients with ACS, particularly those patients who presented early after symptom onset.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE60146
Description	This Human Thrombus precursor protein (TpP) ELISA Kit employs a two-site sandwich ELISA to quantitate TpP.
Alternative Names	TpP
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Thrombus precursor protein microplate Human Thrombus precursor protein standard Human Thrombus precursor protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Thrombus precursor protein (TpP) ELISA Kit has high sensitivity and excellent specificity for detection of Human TpP. No significant cross-reactivity or interference between Human TpP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Thrombus precursor protein (TpP), which measures soluble fibrin, is a marker of active thrombosis. Patients with ACS who present early after symptom onset have been shown to have significantly higher levels of TpP as compared to controls. Several smaller studies have evaluated TpP as a diagnostic tool and found that TpP appears to offer useful information for patients with ACS, particularly those patients who presented early after symptom onset.
Notes	This Human Thrombus precursor protein (TpP) ELISA Kit employs a two-site sandwich ELISA to quantitate TpP in samples. An antibody specific for TpP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTpP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TpP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TpP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Antigen	TpP
Tag	TpP
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Thrombus precursor protein (TpP) ELISA Kit employs a two-site sandwich ELISA to quantitate TpP in samples. An antibody specific for TpP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTpP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TpP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TpP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
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