Human Thrombopoietin,TPO ELISA kit

Mfr.No.

CSB-E04745h

Sensitivity

1.56 pg/mL

Detection Range

6.25 pg/mL-400 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

thrombopoietin, MGC163194, MGDF, MKCSF, ML, MPLLG, TPO, MPL ligand, c-mpl ligand, megakaryocyte colony-stimulating factor, megakaryocyte growth and development factor, megakaryocyte stimulating factor, myeloproliferativ

Recovery

The recovery of human SNCA oligomer spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-97
EDTA plasma (n=4)	93	90-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SNCA oligomer in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

82-94

1:2

Average %

Range %

87-99

1:4

Average %

Range %

90-102

1:8

Average %

102

Range %

96-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.368	2.277	2.323	2.161
10	1.813	1.905	1.859	1.697
5	1.198	1.164	1.181	1.019
2.5	0.772	0.801	0.787	0.625
1.25	0.451	0.434	0.443	0.281
0.625	0.328	0.316	0.322	0.160
0.312	0.241	0.233	0.237	0.075
0	0.163	0.161	0.162	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P40225

Applications	ELISA
Research Area	THPO

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504550	CSB-E04745h	96T	$595	Add to Cart
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BHE10504550	CSB-E04745h	10x96T	$5652.50	Add to Cart

Human Thrombopoietin,TPO ELISA kit

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