Human thrombin-antithrombin complex,TAT ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08431h

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

thrombin-antithrombin complex,TAT

Recovery

The recovery of mouse Hyp spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-101
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hyp in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	89
	Range %	82-97
1:40	Average %	98
	Range %	91-104
1:80	Average %	98
	Range %	96-105
1:160	Average %	97
	Range %	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	TAT

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effects of freeze-thawing and intravenous infusion on mesenchymal stromal cell gene expression Hoogduijn MJ.et al,Stem Cells Dev.,2016 Feb 25. Switching biological functionalities of biointerfaces via dynamic covalent bonds Jie Deng. et al,Journal of Materials Chemistry B,2016 4, 694-703

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