Human Thiosulfate sulfurtransferase(TST) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL025197HU

Alternative Names

thiosulfate sulfurtransferase (rhodanese), MGC19578, RDS, rhodanese, thiosulfate sulfurtransferase

Reactivity

Human

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of chicken Hsp-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	96	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken Hsp-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-97
1:2	Average %	91
	Range %	85-98
1:4	Average %	95
	Range %	91-99
1:8	Average %	93
	Range %	84-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q16762
Antigen	thiosulfate sulfurtransferase (rhodanese)

Applications

ELISA

Research Area

TST

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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