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Home / ELISA Kit

Human Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit

SKU : BHE13702457

TMX4, a type I transmembrane protein, was localized to the ER and possessed a Trx-like domain that faced the ER lumen. A maleimide alkylation assay showed that a catalytic CXXC motif in the TMX4 Trx-like domain underwent changes in its redox state depending on cellular redox conditions, and, in the normal state, most of the endogenous TMX4 existed in the oxidized form. The redox potential of this domain indicated that TMX4 could work as a reductase in the environment of the ER. TMX4 had no effect on the acceleration of ER-associated degradation. Because TMX4 interacted with calnexin and ERp57 by co-immunoprecipitation assay, the role of TMX4 may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER.

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Mfr.No.	KTE60239
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit employs a two-site sandwich ELISA to quantitate TMX4.
Alternative Names	TMX4; DJ971N18.2; KIAA1162; PDIA14; TXNDC13; protein disulfide isomerase family A; member 14; thioredoxin domain containing 13
Detection Method	Colorimetric
Kit Components	Human Thioredoxin-related transmembrane protein 4 microplate Human Thioredoxin-related transmembrane protein 4 standard Human Thioredoxin-related transmembrane protein 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMX4. No significant cross-reactivity or interference between Human TMX4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TMX4, a type I transmembrane protein, was localized to the ER and possessed a Trx-like domain that faced the ER lumen. A maleimide alkylation assay showed that a catalytic CXXC motif in the TMX4 Trx-like domain underwent changes in its redox state depending on cellular redox conditions, and, in the normal state, most of the endogenous TMX4 existed in the oxidized form. The redox potential of this domain indicated that TMX4 could work as a reductase in the environment of the ER. TMX4 had no effect on the acceleration of ER-associated degradation. Because TMX4 interacted with calnexin and ERp57 by co-immunoprecipitation assay, the role of TMX4 may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER.
Notes	This Human Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit employs a two-site sandwich ELISA to quantitate TMX4 in samples. An antibody specific for TMX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMX4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	56255

Applications	ELISA
Applications Notes	This Human Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit employs a two-site sandwich ELISA to quantitate TMX4 in samples. An antibody specific for TMX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMX4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702457	KTE60239	48 T	$339		Add to Cart
BHE13702457	KTE60239	96 T	$589		Add to Cart
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BHE13702457	KTE60239	96 Tx50	$24569		Add to Cart

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