Human Thioredoxin domain-containing protein 5(TXNDC5) ELISA kit

Mfr.No.

CSB-EL025379HU

Alternative Names

thioredoxin domain containing 5 (endoplasmic reticulum), ERP46, EndoPDI, Hcc-2, MGC3178, PDIA15, UNQ364, endothelial protein disulphide isomerase, protein disulfide isomerase family A, member 15, thioredoxin domain containing 5, thioredoxin related protein

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Duration

1-5h

Recovery

The recovery of human PLXNB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	96-107
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PLXNB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	87
	Range %	82-91
1:4	Average %	96
	Range %	92-100
1:8	Average %	90
	Range %	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.543	2.446	2.495	2.389
2500	1.619	1.534	1.577	1.471
1250	0.872	0.921	0.897	0.791
625	0.508	0.520	0.514	0.408
312	0.335	0.318	0.327	0.221
156	0.217	0.205	0.211	0.105
78	0.151	0.159	0.155	0.049
0	0.104	0.107	0.106	?

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q8NBS9
Antigen	thioredoxin domain containing 5 (endoplasmic reticulum)

Applications

ELISA

Research Area

TXNDC5

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504538	CSB-EL025379HU	96T	$695	Add to Cart
BHE10504538	CSB-EL025379HU	5x96T	$3336	Add to Cart
BHE10504538	CSB-EL025379HU	10x96T	$6602.50	Add to Cart

Human Thioredoxin domain-containing protein 5(TXNDC5) ELISA kit

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