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Home / ELISA Kit

Human Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) ELISA Kit

SKU : BHE13703397

PRDX3 encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.

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Mfr.No.	KTE61181
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX3.
Alternative Names	PRDX3; AOP-1; AOP1; MER5; MGC104387; MGC24293; PRO1748; SP-22; antioxidant protein 1; thioredoxin-dependent peroxide reductase
Detection Method	Colorimetric
Kit Components	Human Thioredoxin-dependent peroxide reductase, mitochondrial microplate Human Thioredoxin-dependent peroxide reductase, mitochondrial standard Human Thioredoxin-dependent peroxide reductase, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRDX3. No significant cross-reactivity or interference between Human PRDX3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PRDX3 encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.
Notes	This Human Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX3 in samples. An antibody specific for PRDX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10935

Applications	ELISA
Applications Notes	This Human Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX3 in samples. An antibody specific for PRDX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703397	KTE61181	48 T	$339		Add to Cart
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