Human TFPI2 ELISA Kit PicoKine®

Mfr.No.

EK1358

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
O.D.	0.023	0.222	0.406	0.673	1.136	1.538	1.824	2.072

Description

Human TFPI2 ELISA Kit PicoKine® (96 Tests). Quantitate Human TFPI2 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tissue factor pathway inhibitor 2;TFPI-2;Placental protein 5;PP5;TFPI2;

Molecular Weight

26934 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D23-F235

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TFPI2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	65	181	1685	89	200	1782
Standard deviation	3.66	8	48.02	2.52	9.28	109.77
CV(%)	5.6%	4.4%	2.9%	2.8%	4.6%	6.2%

Protein Function

May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin. .

Background

TFPI2, also called Tissue factor pathway inhibitor 2, is a protein that in humans is encoded by the TFPI2 gene. It is mapped to 7q21.3. This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Qualitative PCR and microarray analyses showed that expression of TFPI2 was inversely correlated with that of MIR616 in prostate cancer cell lines, primary tumors of various grades, and normal tissue.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	65	78	115	122	95	6.06	6.4%
Sample 2	181	159	165	184	172	8.39	4.9%
Sample 3	1685	1837	1867	1152	1635	43.99	2.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7980
Gene Name	TFPI2
UniProt ID	P48307
Protein Name	Tissue factor pathway inhibitor 2
Tissue Specificity	Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and mateal serum at advanced pregnancy.

Applications	ELISA
Research Area	Cardiovascular,Cell Biology,Protease Inhibitors,Proteolysis/Ubiquitin

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000952	EK1358	96 wells/kit	$499		Add to Cart

Human TFPI2 ELISA Kit PicoKine®

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