Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine®

Mfr.No.

EK0840

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.043	0.069	0.119	0.167	0.283	0.515	0.944	1.764

Description

Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine® (96 Tests). Quantitate Human TFPI in cell culture supeatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tissue factor pathway inhibitor;TFPI;Extrinsic pathway inhibitor;EPI;Lipoprotein-associated coagulation inhibitor;LACI;TFPI;LACI, TFPI1;

Molecular Weight

35015 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D29-K282

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TFPI

Subcellular Localization

Isoform Alpha: Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	57	238	848	55	237	897
Standard deviation	3.3	11.9	66.14	3.46	14.93	75.34
CV (%)	5.8%	5%	7.8%	6.3%%	6.3%	8.4%

Protein Function

Inhibits factor X (X (a)) ly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quateary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

Background

TFPI (Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus.TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	57	57	66	64	61	4.06	6.6%
Sample 2	238	272	240	245	248	13.66	5.5%
Sample 3	848	982	998	845	918	71.98	7.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7035
Gene Name	TFPI
UniProt ID	P10646
Protein Name	Tissue factor pathway inhibitor
Tissue Specificity	Mostly in endothelial cells.
Sequence	Contains 3 BPTI/Kunitz inhibitor domains.

Applications	ELISA
Research Area	Cardiovascular,Platelets

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000649	EK0840	96 wells/kit	$499		Add to Cart

Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine®

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