Matrices listed below were spiked with certain level of TFF2 and the recovery rates were calculated by comparing the measured value to the expected amount of TFF2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
94
EDTA plasma(n=5)
89-105
97
heparin plasma(n=5)
89-98
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TFF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
TFF2 ELISA Kit allows for the in vitro quantitative determination of TFF2 concentrations in serum, plasma, tissue homogenates and other biological fluids.