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Home / ELISA Kit

Human Testosterone (T) ELISA Kit

SKU : BHE13702665

Testosterone is the primary male sex hormone and an anabolic steroid. In men, testosterone plays a key role in the development of male reproductive tissues such as the testis and prostate, as well as promoting secondary sexual characteristics such as increased muscle and bone mass, and the growth of body hair. In addition, testosterone is involved in health and well-being, and the prevention of osteoporosis. Insufficient levels of testosterone in men may lead to abnormalities including frailty and bone loss.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE60448
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Testosterone (T) ELISA Kit employs a two-site sandwich ELISA to quantitate T.
Alternative Names	T; RP11-459F1.1; MGC104817; TFT; OTTHUMP00000017588; T brachyury homolog; T brachyury-like; transcription factor T
Detection Method	Colorimetric
Kit Components	Human Testosterone microplate Human Testosterone standard Human Testosterone detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Testosterone (T) ELISA Kit has high sensitivity and excellent specificity for detection of Human T. No significant cross-reactivity or interference between Human T and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Testosterone is the primary male sex hormone and an anabolic steroid. In men, testosterone plays a key role in the development of male reproductive tissues such as the testis and prostate, as well as promoting secondary sexual characteristics such as increased muscle and bone mass, and the growth of body hair. In addition, testosterone is involved in health and well-being, and the prevention of osteoporosis. Insufficient levels of testosterone in men may lead to abnormalities including frailty and bone loss.
Notes	This Human Testosterone (T) ELISA Kit employs a two-site sandwich ELISA to quantitate T in samples. An antibody specific for T has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for T is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of T bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Testosterone (T) ELISA Kit employs a two-site sandwich ELISA to quantitate T in samples. An antibody specific for T has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for T is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of T bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702665	KTE60448	48 T	$379		Add to Cart
BHE13702665	KTE60448	96 T	$719		Add to Cart
BHE13702665	KTE60448	96 Tx5	$3159		Add to Cart
BHE13702665	KTE60448	96 Tx50	$28079		Add to Cart

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