Matrices listed below were spiked with certain level of TESPA1 and the recovery rates were calculated by comparing the measured value to the expected amount of TESPA1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-97
94
EDTA plasma(n=5)
87-103
95
heparin plasma(n=5)
86-105
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TESPA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
87-104%
85-95%
EDTA plasma(n=5)
86-98%
84-101%
89-98%
heparin plasma(n=5)
82-100%
80-100%
82-92%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
A2RU30
Applications
ELISA
Applications Notes
TESPA1 ELISA Kit allows for the in vitro quantitative determination of TESPA1 concentrations in serum, plasma, tissue homogenates and other biological fluids.