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Home / ELISA Kit

Human Tensin-3 (TNS3) ELISA Kit

SKU : BHE13702392

Tensin-3 formed a complex with focal adhesion kinase and p130Cas (BCAR1) in human breast carcinoma cells. Addition of EGF to these cells induced dephosphorylation of FAK and p130Cas, leading to dissociation of the tensin-3/FAK/p130Cas complex and enhanced interaction between tensin-3 and EGFR. The deduced 1,445-amino acid protein has a calculated molecular mass of 155.3 kD. Like other tensins, tensin-3 has an N-terminal actin-binding domain and C-terminal SH2 and PTB domains. It also has several potential tyrosine phosphorylation sites. Northern blot analysis detected high expression of an 8-kb transcript in kidney and placenta, with weaker expression in heart, skeletal muscle, spleen, liver, and lung. Western blot analysis revealed a 180-kD tensin-3 protein in human endothelial and epithelial cells and fibroblasts.

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Mfr.No.	KTE60173
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tensin-3 (TNS3) ELISA Kit employs a two-site sandwich ELISA to quantitate TNS3.
Alternative Names	TNS3; DKFZp686K12123; DKFZp686M1045; FLJ13732; FLJ35545; H_NH049I23.2; MGC88434; TEM6; TENS1; H_NH0549I23.2; OTTHUMP00000082709; tensin-like SH2 domain containing 1; tensin-like SH2 domain-containing 1; thyro
Detection Method	Colorimetric
Kit Components	Human Tensin-3 microplate Human Tensin-3 standard Human Tensin-3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tensin-3 (TNS3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNS3. No significant cross-reactivity or interference between Human TNS3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tensin-3 formed a complex with focal adhesion kinase and p130Cas (BCAR1) in human breast carcinoma cells. Addition of EGF to these cells induced dephosphorylation of FAK and p130Cas, leading to dissociation of the tensin-3/FAK/p130Cas complex and enhanced interaction between tensin-3 and EGFR. The deduced 1,445-amino acid protein has a calculated molecular mass of 155.3 kD. Like other tensins, tensin-3 has an N-terminal actin-binding domain and C-terminal SH2 and PTB domains. It also has several potential tyrosine phosphorylation sites. Northern blot analysis detected high expression of an 8-kb transcript in kidney and placenta, with weaker expression in heart, skeletal muscle, spleen, liver, and lung. Western blot analysis revealed a 180-kD tensin-3 protein in human endothelial and epithelial cells and fibroblasts.
Notes	This Human Tensin-3 (TNS3) ELISA Kit employs a two-site sandwich ELISA to quantitate TNS3 in samples. An antibody specific for TNS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	64759

Applications	ELISA
Applications Notes	This Human Tensin-3 (TNS3) ELISA Kit employs a two-site sandwich ELISA to quantitate TNS3 in samples. An antibody specific for TNS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702392	KTE60173	48 T	$339		Add to Cart
BHE13702392	KTE60173	96 T	$589		Add to Cart
BHE13702392	KTE60173	96 Tx5	$2629		Add to Cart
BHE13702392	KTE60173	96 Tx50	$24569		Add to Cart

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