This Human FGF8 ELISA Kit was designed for the quantitative measurement of Human FGF8 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
The recovery of human FGF8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
89-98
EDTA plasma (n=4)
97
90-100
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FGF8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
91
Range %
86-95
1:2
Average %
102
Range %
97-107
1:4
Average %
91
Range %
85-97
1:8
Average %
97
Range %
91-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1000
2.455
2.498
2.477
2.290
500
1.872
1.932
1.902
1.715
250
1.213
1.236
1.225
1.038
125
0.687
0.699
0.693
0.506
62.5
0.499
0.484
0.492
0.305
31.2
0.379
0.398
0.389
0.202
15.6
0.277
0.287
0.282
0.095
0
0.188
0.186
0.187
Species
Homo sapiens
Sample Type
Serum, Tissue Homogenates, Plasma
Limit Of Detection
450 nm
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.