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Home / ELISA Kit

Human TCDD-inducible poly [ADP-ribose] polymerase (TIPARP) ELISA Kit

SKU : BHE13702539

TIPARP, encodes a deduced 657-amino acid protein that shares 91.8% and 52.7% sequence identity with mouse and fugu Tiparp proteins, respectively. The proteins in all 3 species contain 3 highly conserved domains: a WWE (trp, trp, glu) domain, a PARP (poly(ADP-ribose) polymerase)-like catalytic domain, and a novel TPH (TIPARP homologous) domain.
The N-terminal part of the TPH domain contains a CCCH-type zinc finger. Katoh and Katoh (2003) noted that the deduced proteins of 2 human genes, which they called FLJ226093 (PARP12) and ZAP (ZC3HAV1), contain the same 3 conserved domains and share 27.5% and 26% overall sequence identity with TIPARP, respectively.

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Mfr.No.	KTE60321
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human TCDD-inducible poly (ADP-ribose) polymerase (TIPARP) ELISA Kit employs a two-site sandwich ELISA to quantitate TIPARP.
Alternative Names	TIPARP; DDF1; DKFZp434J214; DKFZp686N0351; DKFZp686P1838; FLJ40466; PARP-1; PARP-7; PARP7;
Detection Method	Colorimetric
Kit Components	Human TCDD-inducible poly (ADP-ribose) polymerase microplate Human TCDD-inducible poly (ADP-ribose) polymerase standard Human TCDD-inducible poly (ADP-ribose) polymerase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human TCDD-inducible poly (ADP-ribose) polymerase (TIPARP) ELISA Kit has high sensitivity and excellent specificity for detection of Human TIPARP. No significant cross-reactivity or interference between Human TIPARP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TIPARP, encodes a deduced 657-amino acid protein that shares 91.8% and 52.7% sequence identity with mouse and fugu Tiparp proteins, respectively. The proteins in all 3 species contain 3 highly conserved domains: a WWE (trp, trp, glu) domain, a PARP (poly(ADP-ribose) polymerase)-like catalytic domain, and a novel TPH (TIPARP homologous) domain. The N-terminal part of the TPH domain contains a CCCH-type zinc finger. Katoh and Katoh (2003) noted that the deduced proteins of 2 human genes, which they called FLJ226093 (PARP12) and ZAP (ZC3HAV1), contain the same 3 conserved domains and share 27.5% and 26% overall sequence identity with TIPARP, respectively.
Notes	This Human TCDD-inducible poly (ADP-ribose) polymerase (TIPARP) ELISA Kit employs a two-site sandwich ELISA to quantitate TIPARP in samples. An antibody specific for TIPARP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIPARP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIPARP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIPARP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	25976

Applications	ELISA
Applications Notes	This Human TCDD-inducible poly (ADP-ribose) polymerase (TIPARP) ELISA Kit employs a two-site sandwich ELISA to quantitate TIPARP in samples. An antibody specific for TIPARP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIPARP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIPARP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIPARP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702539	KTE60321	48 T	$339		Add to Cart
BHE13702539	KTE60321	96 T	$589		Add to Cart
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BHE13702539	KTE60321	96 Tx50	$24569		Add to Cart

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