Matrices listed below were spiked with certain level of TCC C5b-9 and the recovery rates were calculated by comparing the measured value to the expected amount of TCC C5b-9 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-99
93
EDTA plasma(n=5)
85-102
96
heparin plasma(n=5)
88-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TCC C5b-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-103%
85-101%
87-105%
EDTA plasma(n=5)
82-99%
84-100%
90-99%
heparin plasma(n=5)
82-97%
86-100%
92-100%
Sensitivity
< 7.5ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
TCC C5b-9 ELISA Kit allows for the in vitro quantitative determination of TCC C5b-9 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human TCC C5b-9 (Terminal Complement Complex C5b-9)ELISA Kit