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Home / ELISA Kit

Human TATA element modulatory factor (TMF1) ELISA Kit

SKU : BHE13702479

The TATA element, or TATA box, is a critical cis-acting regulatory element in many promoters transcribed by RNA polymerase II. The RNA polymerase II transcription factor D is a biochemically defined HeLa cell nuclear fraction containing a transcription factor activity that binds specifically to the TATA box. The DNA-binding subunit of TFIID is the TATA box-binding protein . TMF binds to the HIV-1 TATA element in gel-retardation assays and inhibits activation of the HIV-1 long terminal repeat by TBP in in vitro transcription assays. Sequence analysis of the TMF cDNA revealed leucine zipper motifs and homology in its DNA-binding domain with the phage-encoded DNA binding protein Ner. In cataloging sequences from a human adult testis expression library, Affara et al. (1994) also identified a cDNA clone for TMF.

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Mfr.No.	KTE60261
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human TATA element modulatory factor (TMF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMF1.
Alternative Names	TMF1; ARA160; TMF;
Detection Method	Colorimetric
Kit Components	Human TATA element modulatory factor microplate Human TATA element modulatory factor standard Human TATA element modulatory factor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human TATA element modulatory factor (TMF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMF1. No significant cross-reactivity or interference between Human TMF1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The TATA element, or TATA box, is a critical cis-acting regulatory element in many promoters transcribed by RNA polymerase II. The RNA polymerase II transcription factor D is a biochemically defined HeLa cell nuclear fraction containing a transcription factor activity that binds specifically to the TATA box. The DNA-binding subunit of TFIID is the TATA box-binding protein . TMF binds to the HIV-1 TATA element in gel-retardation assays and inhibits activation of the HIV-1 long terminal repeat by TBP in in vitro transcription assays. Sequence analysis of the TMF cDNA revealed leucine zipper motifs and homology in its DNA-binding domain with the phage-encoded DNA binding protein Ner. In cataloging sequences from a human adult testis expression library, Affara et al. (1994) also identified a cDNA clone for TMF.
Notes	This Human TATA element modulatory factor (TMF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMF1 in samples. An antibody specific for TMF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7110

Applications	ELISA
Applications Notes	This Human TATA element modulatory factor (TMF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMF1 in samples. An antibody specific for TMF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702479	KTE60261	48 T	$339		Add to Cart
BHE13702479	KTE60261	96 T	$589		Add to Cart
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BHE13702479	KTE60261	96 Tx50	$24569		Add to Cart

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