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Home / ELISA Kit

Human Taste receptor type 2 member 20 (TAS2R20) ELISA Kit

SKU : BHE13702709

Taste receptor type 2 member 20 (TAS2R20) is a member of the taste receptor two family of class C G-protein coupled receptors. Receptors of this family have a short extracellular N-terminus, seven transmembrane helices, three extracellular loops and three intracellular loops, and an intracellular C-terminus. Members of this family are expressed in a subset of taste receptor cells, where they function in bitter taste reception, as well as in non-gustatory cells including those of the brain, reproductive organs, respiratory system, and gastrointestinal system. TAS2R20 maps to the taste receptor gene cluster on chromosome 12p13.

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Mfr.No.	KTE60492
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Taste receptor type 2 member 20 (TAS2R20) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS2R20.
Alternative Names	TAS2R20; MGC119118; MGC119119; MGC119120; MGC119121; MGC134637; T2R20; T2R49; T2R56; TAS2R49; taste receptor; type 2; member 49
Detection Method	Colorimetric
Kit Components	Human Taste receptor type 2 member 20 microplate Human Taste receptor type 2 member 20 standard Human Taste receptor type 2 member 20 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Taste receptor type 2 member 20 (TAS2R20) ELISA Kit has high sensitivity and excellent specificity for detection of Human TAS2R20. No significant cross-reactivity or interference between Human TAS2R20 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Taste receptor type 2 member 20 (TAS2R20) is a member of the taste receptor two family of class C G-protein coupled receptors. Receptors of this family have a short extracellular N-terminus, seven transmembrane helices, three extracellular loops and three intracellular loops, and an intracellular C-terminus. Members of this family are expressed in a subset of taste receptor cells, where they function in bitter taste reception, as well as in non-gustatory cells including those of the brain, reproductive organs, respiratory system, and gastrointestinal system. TAS2R20 maps to the taste receptor gene cluster on chromosome 12p13.
Notes	This Human Taste receptor type 2 member 20 (TAS2R20) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS2R20 in samples. An antibody specific for TAS2R20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS2R20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS2R20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS2R20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	259295

Applications	ELISA
Applications Notes	This Human Taste receptor type 2 member 20 (TAS2R20) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS2R20 in samples. An antibody specific for TAS2R20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS2R20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS2R20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS2R20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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